Polimer Kitosan-Natrium Tripolifosfat Tercetak Molekul: Sintesis dan Aplikasinya untuk Ekstraksi Residu Antibiotik pada Hasil Peternakan
Abstrak
Analisis residu antibiotik menjadi suatu tantangan karena rendahnya konsentrasi dalam matriks barang pertanian yang rumit. Dalam penelitian ini, adsorben selektif yang dikembangkan dari polimer tercetak secara molekuler (MIP) digunakan secara efektif untuk preparasi sampel. Melalui penggunaan polimer tercetak molekul berbasis kitosan(Chi-MIP) , residu tetrasiklin secara efektif diekstraksi dari sampel telur dan susu. Adsorben diabuat dengan memasukkan 0.1 gram Chi-MIP ke dalam kantong yang terbuat dari kertas saring selulosa ukuran 2 x 2 cm. Adsorben dimasukkan ke dalam larutan sampel yang mengandung tetrasiklin. Proses ekstraksi dilakukan dengan menggunakan pengaduk hot plate. Pada akhir proses ekstraksi, antibiotik yang diekstraksi dikeluarkan dari adsorben melalui proses desorpsi dalam pelarut organik menggunakan ultrasonikator. Setelah itu, analit yang terdesorbsi dianalisis menggunakan spektrofotometer UV-Vis pada panjang gelombang 267 nm. Untuk memperoleh hasil optimum, kondisi ekstraksi dioptimasi sehingga diperoleh hasil sebagai berikut: waktu ekstraksi 12 menit, waktu desorpsi 3 menit, dan etil asetat sebagai pelarut organik pendesorbsi. Melalui metode standar adisi, diperoleh hasil validasi metode pada sampel susu dan telur masing-masing menunjukkan rentang linearitas 1–5 mgL-1, koefisien korelasi (R2) sebesar 0.99, nilai akurasi sebesar 98.22% dan 88.10% , presisi sebesar 2.74% dan 1.06%, LoD sebesar 0.46 mgL-1 dan 0.51 mgL-1, LoQ sebesar 1.52 mgL-1 dan 1.70 mgL-1, serta faktor pengayaan sebesar 3.27 kali.
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